Objetivo Comparar las coloraciones Ziehl-Neelsen modificada (ZNm) y Safranina como la coloración Safranina modificada la cual ha mostrado una alta. Tinción de Ziehl-Neelsen. 2. Digestión y variante de Kinyoun o coloración fría , emplea cuerpos parece que en sensibilidad supere a la tinción de Ziehl-. COLORACION ZIEHL NEELSEN – Download as Powerpoint Presentation .ppt /. pptx), PDF File .pdf), Text File .txt) or view presentation slides online.

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Retrieved from ” https: From Wikipedia, the free encyclopedia. Applications to Patient Care.

When counter stain is applied, non-acid-fast bacteria pick it up and become blue methylene blue or green malachite green when viewed under the microscope.

Views Read Edit View history. How to identify fungi to genus III: It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.

Journal of Clinical Pathology. Laboratory Manual and Colkracion in Microbiology: The Ziehl—Neelsen stainalso known as the acid-fast stainwas first described by two German doctors: Other important Mycobacterium species involved in human disease are Mycobacterium lepraeMycobacterium kansasiiMycobacterium marinumMycobacterium bovisMycobacterium africanum and members of the Mycobacterium avium complex.


McGraw-Hill Higher Education, Acid-fast organisms like Mycobacterium contain large amounts of lipid substances within their cell walls called mycolic acids.

Thereafter, the cells are stained in methylene blue and viewed under a microscope coloracin oil immersion.

A variation on this staining method is used in mycology to differentially stain acid-fast incrustations in the cuticular hyphae of certain species of fungi in the coloracionn Russula. The slide is flooded with carbol fuchsin, which is then heated to dry and rinsed off in tap water. Please help improve this article by adding citations to reliable sources. Mycobacterium tuberculosis is the most important coloraciom this group because it is responsible for tuberculosis TB.

Ziehl–Neelsen stain

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This page was last edited on 14 Decemberat A typical AFB stain procedure involves dropping the cells in suspension onto a slide, then air drying the liquid and heat fixing the cells. Wilson, and Verna Morton. The Ziehl—Neelsen stain can also hinder diagnosis in the case of paragonimiasis because the eggs in an ovum and parasite sputum sample OnP can be dissolved by the stain, and is often used in this clinical setting because signs and symptoms of paragonimiasis closely resemble those of TB.


Les Russules d’Europe et d’Afrique du Nord. The reagents used for Ziehl—Neelsen staining are — carbol fuchsinacid alcohol, and methylene blue.

Ziehl–Neelsen stain – Wikipedia

Unsourced material may be challenged and removed. Microbial and histological stains. Acid-fast bacteria retain carbol fuchsin so they appear red. This article needs additional citations for verification.

Coloración Gram y Ziehl Neelsen by Jennifer Yaquí on Prezi

These acids resist staining by ordinary methods such as ziebl Gram stain. Initially, carbol fuchsin stains every cell. Acid-fast bacilli are bright red after staining. Studies have shown that an AFB stain without a culture has a poor negative predictive value. When they are de-stained with acid-alcohol, only non-acid-fast bacteria get de-stained since they do not have a thick, waxy lipid layer like acid-fast bacteria.